Brief Introduction of Streptavidin-RPE

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This streptavidin-RPE conjugate comprises streptavidin (as the biotin-binding protein) with RPE covalently attached (as the fluorescent label).

It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry, microplate reader and microarray platforms.